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. 2020 May 14;20:115. doi: 10.1186/s12866-020-01789-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Structural arrangement between exopolysaccharide (EPS) and extracellular DNA (eDNA) during biofilm development of EPS mutant (∆epsG). A Representative 3D renderings of B. subtilis SBE1 biofilms at 12, 24 and 48 h: (a) shows the dynamic evolution of biofilms over time. Panel (b-J) show cross sectional images of selected area for close-up views of structural organization of EPS (orange) and eDNA (green) during the development of biofilm matrix complex. B The biomass values of EPS and eDNA in the biofilms were calculated using Imaris. The data shown are mean values ± SD (n = 3)