Table 1.

Primers used for analysis of mRNA expression using reverse transcriptase (RT) and real-time quantitative PCR. Primers of Nox family were obtained from GeneCopoeia™ (Rockville, MD) and as previously described [28]. Primers of genes mediating EMT were designed with NIH primer blast (https://www.ncbi.nlm.nih. gov/tools/primer-blast) based on genes sequences obtained from the Gen Bank NCBI sequence Viewer.

Gene	Forward Primer (5’ to 3’)	Reverse Primer (5’ to 3’)	Accession Number	PCR product size
Nox enzymes
Nox1	GGAGTGGCATCCCTTCACTC	GGCATTGGTGAGTGCTGTTG	NM_172203.2	127 bp
Nox1 (GeneCopoeia)	MQP097901	MQP097901	NM_172203.2	132 bp
Nox2	CGAGTCACGGCCACATACA	TGAATGCCAGAGTCGGGATTT	FJ168469.1	198 bp
Nox3	CTCGTTGCCTACGGGATAGC	CCTTCAGCATCCTTGGCCT	NM_198958.2	184 bp
Nox4	MQP078234	MQP078234	NM_001285835	147 bp
Genes mediating EMT
Tgf-P	MQP030343	MQP030343	NM_011577.1	125 bp
Slug or snail family zinc finger 2 (Snai2)	CTGTATGGACATCGTCGGCA	ACTTACACGCCCCAAGGATG	NM_011415.3	94 bp
Zinc finger E-box binding homeobox 1 (Zeb1), transcript variant 1	GGTACAGTGGAGGTTGGAGC	CTCTCGCTTTCGCTCTCCAT	NM_011546.3	85 bp
Zinc finger E-box binding homeobox 2 (Zeb2), transcript variant 2	CATGCCCAACCATGAGTCCT	TTCTGGCCCCATTGCATCAT	NM_015753.4	177 bp
Twist basic helix-loop-heUx transcription factor 1 (twist1)	ATTCAGACCCTCAAACTGGCG	TCTTGGAGTCCAGCTCGTCG	NM_011658.2	78 bp
Matrix metalloproteinase 2 (MMP-2)	TGTACAGACACTGGTCGCAG	AAACAAGGCTTCATGGGGGC	NM_008610.3	101 bp
Matrix metalloproteinase 9 (MMP-9)	CGACTTTTGTGGTCTTCCCCA	AGGTTTGGAATCGACCCACG	NM_013599.4	254 bp
Snail family zinc finger 1 (Snai1)	ACCTCCAAACCCACTCGGAT	GACATGCGGGAGAAGGTTCG	NM_011427.3	69 bp
Housekeeping gene
18S rRNA	GTAACCCGTTGAACCCCATT	CCATCCAATCGGTAGTAGCG	NR_003278.3	150 bp