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. 2020 Apr 29;16:1744806920919568. doi: 10.1177/1744806920919568

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Cellular colocalization of CXCR4 with p-RhoA and p-ROCK2 in rat spinal dorsal neurons. (a to d) Double immunofluorescence staining showing cellular colocalization of p-RhoA/p-ROCK2 and cell markers in TCI rat spinal cord. p-RhoA (green, a) and p-ROCK2 (green, c) were co-localized with neurons (NeuN, red). Meanwhile, p-RhoA (green, b) and p-ROCK2 (green, d) were not co-expressed with astrocytes (GFAP, red) or microglial cells (IBA-1, red). L4 to L6 spinal tissues were taken on day 14 after TCI. Original magnification: 400× (a to d) and 1600× (insets in a to d); scale bar: 50 μm (d to g) and 20 μm (insets in a to d). GFAP: glial fibrillary acidic protein; IBA-1: ionized calcium binding adapter molecule-1.