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. 2020 Apr 29;16:1744806920919568. doi: 10.1177/1744806920919568

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Intrathecal (i.t.) injection of Fasudil or AMD3100 attenuates TCI-induced upregulation of p-ROCK2, p-RhoA, and CXCR4 expressions (a and d). (a and c) Representative bands and (b and d) quantitative data. Intrathecal injection of Fasudil attenuates TCI-induced upregulation of p-ROCK2 expression, but not TCI-induced upregulation of p-RhoA and CXCR4. AMD3100 attenuates the TCI-induced upregulation of p-ROCK2, p-RhoA, and CXCR4 expressions. Western blot analysis showing the inhibitory effect of Fasudil and AMD3100 on TCI-induced overexpressions of p-ROCK2, p-RhoA, and CXCR4 on day 7 after TCI. Fasudil (20 μg/10 μL), AMD3100 (5 μg/10 μL), or PBS (10 μL) was i.t. administered once daily on days 5, 6, and 7 (a and b) after TCI. L4 to L6 spinal tissues were collected 12 h after the last injection. *P < 0.05 vs sham group; **P < 0.01 versus sham group; ^#P < 0.05,^##P < 0.01 versus TCI group; n = 4 for each group. TCI: tumor cell implantation; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; CXCR4: chemokine C–X–C motif receptor 4.