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. 2019 May 8;20(Suppl 3):298. doi: 10.1186/s12864-019-5538-z

Table 1.

Primer sequences used for amplification of different LINC01420 loci and reference genes and siRNA sequences used for knockdown

Primer Sequence
F1 5′ - GCCCACCGGAGAAAACTGAC – 3
R1 5′ – CCTTCCGGATAATCCCAACG - 3‘
R1–1 5′ – GAGGCTTGGCTTCCCGTG - 3‘
R1–2 5′ – TTCCAGGTGGGAGAGTGGA – 3
F2 5′ – AGTGATTGCAGTATGACTCCA - 3‘
R2 5′ – TTCCAGGTTCAGGACACCAGA - 3‘
F3 5′ – TCAGCGCGATTTCACTTCCTG – 3’
F3–1 5′ – CTGAATTTCGATGAATTCTAAGAC – 3’
F3–2 5′ – ACCTCTGAGATTTAAGGCCATG – 3’
R3 5′ – GACCATCTCACAGGCATTGTT – 3’
F4 5′ – GTACACTTTCTTTAATTTGCTGTC – 3’
F5 5′ – GAAAATGTCAGATAAACTTGGCT – 3’
R5 5′ – CTTTAATTCTGATGCTAGGGACT – 3’
HPRTf 5′ – TGTAATGACCAGTCAACAGGG - 3’
HPRTr 5′ – TGCGACCTTGACCATCTTTG - 3’
B2Mf 5′ – TCTTTCAGCAAGGACTGGTC - 3’
B2Mr 5′ – GGCATCTTCAAACCTCCATG - 3’
TBPf 5′ – CGGAGAGTTCTGGGATTGTAC - 3’
TBPr 5′ – GTGGTTCGTGGCTCTCTTATC - 3’
TFRCf 5′ – TCCTTGCATATTCTGGAATCCC - 3’
TFRCr 5′ – ATCACGAACTGACCAGCG - 3’
siLINC01420

5′-UCCGGAGAAGUAGAGAAAUdTdT-3′/

5′-AUUUCUCUACUUCUCCGGAdTdT-3′

siEIF3D#1

5′-GCGUCAUUGACAUCUGCAUdTdT-3′/

5′-AUGCAGAUGUCAAUGACGCdTdT-3′

siEIF3D#2

5′-CGACAUGGAUAAGAAUGAAdTdT-3′/

5′-TTCATTCTTATCCATGTCGdTdT-3’

siControl

5′-AGGUAGUGUAAUCGCCUUGdTdT-3′/

5′-CAAGGCGAUUACACUACCUdTdT-3′

FAM-control

5′-FAM-AGGUCGAACUACGGGUCAAdTdT-3′/

5′-FAM- UUGACCCGUAGUUCGACCUdTdT-3′