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. 2020 May 15;11:179. doi: 10.1186/s13287-020-01701-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Lentiviral-mediated gene expression in IMO iPSC-derived monocytes. Schematic representation of the experimental design (a). IMO iPSCs were transduced with a lentiviral vector expressing TCIRG1 and containing the CBX3-UCOE element (b). EBs were generated from the iPSCs, transferred to tissue culture plates after 4 days of culture, and differentiated in X-VIVO media supplemented with M-CSF and IL-3. The presence of GFP⁺ cells was verified throughout the culture at iPSC stage (c, d), at EB stage (e), and during EB differentiation (f)