Fig. 2.

Effect of miR-193a expression on the proliferation and radiation sensitivity of the medulloblastoma cells evaluated by the MTT assay and the cell cycle analysis by the flow cytometry assay. Medulloblastoma cells expressing the parental pTRIPZ vector alone (Vector control) and the P1/P2 populations expressing doxycycline-inducible miR-193a-pTRIPZ construct were treated with doxycycline before evaluation by the MTT assay or the flow cytometry analysis. a Growth curves of the indicated medulloblastoma cells evaluated by the MTT assay. b Cell cycle analysis of indicated medulloblastoma cells, stained with Propidium iodide and, evaluated by flow cytometry. c Western blot analysis of PARP, a marker of apoptotic cell death, in the indicated medulloblastoma cells. The blot was also probed with anti-GAPDH antibody to serve as a loading control. d Y-axis denotes the surviving fraction of the indicated medulloblastoma cells upon irradiation at a dose ranging from 2 Gy to 6 Gy. VC: Vector control; **, *** and ns indicate p < 0.001, p < 0.0001, respectively