Table 1.
Overview of the NP properties
| NP formulation | After purification | After lyophilization | EE (%) | LC (%) | PVA (%) | |||
|---|---|---|---|---|---|---|---|---|
| dH (nm) | PDI | dH (nm) | PDI | ζ (mV) | ||||
| Acdex | 210 ± 26 | 0.17 ± 0.07 | 211 ± 35 | 0.26 ± 0.09 | − 12 ± 2 | – | – | 0.01 ± 0.000 |
| Acdex[BRP-187] | 196 ± 51 | 0.16 ± 0.11 | 178 ± 26 | 0.13 ± 0.07 | − 13 ± 8 | 59 ± 23 | 1.7 ± 0.6 | 0.01 ± 0.000 |
| Acdex-RhodB[BRP-187] | 163 ± 15 | 0.20 ± 0.06 | 189 ± 37 | 0.28 ± 0.11 | − 24 ± 2 | 67 ± 10 | 2.0 ± 0.3 | n.m. |
| PLGA | 124 ± 6 | 0.06 ± 0.03 | 130 ± 2 | 0.09 ± 0.03 | − 20 ± 2 | – | – | 0.02 ± 0.002 |
| PLGA[BRP-187] | 153 ± 41 | 0.17 ± 0.14 | 158 ± 35 | 0.12 ± 0.06 | − 15 ± 3 | 76 ± 22 | 2.2 ± 0.6 | 0.03 ± 0.002 |
| PLGA-DY635 | 143 ± 3 | 0.08 ± 0.03 | 154 ± 5 | 0.15 ± 0.02 | − 20 ± 1 | – | – | 0.02 ± 0.006 |
| PLGA-DY635[BRP-187] | 153 ± 2 | 0.12 ± 0.01 | 168 ± 9 | 0.19 ± 0.05 | − 19 ± 1 | 87 ± 6 | 2.5 ± 0.2 | 0.02 ± 0.002 |
Concentration of NPs used for the PVA assay was 3 mg mL−1. SD for all measurements n ≥ 3. n.m.–not measured
dH Hydrodynamic diameter obtained by DLS measurements. EE encapsulation efficiency. LC loading capacity