Table 2.
List of combinations of T. gondii recombinant antigens used for the serodiagnosis of human toxoplasmosis.
| Antigen mixture | Expression systema | Diagnostic test | No. and details of tested sera | Results | References |
|---|---|---|---|---|---|
| GRA7-Tg34AR | Recombinant proteins with His tag domain | IgG /IgM ELISA | 180 total, 95 acute, 85 chronic | Sensitivity: 96% (chronic sera) | Jacobs et al., 1999 |
| P29 (GRA7), P30 (SAG1), P35 (GRA8) | Recombinant proteins with CKS domain | IgG ELISA | 247 total; 89 acute, 105 chronic, 53 recent seroconversion |
Sensitivity: 93.1%; specificity, 95.7% | Aubert et al., 2000 |
| P29 (GRA7), P35 (GRA8), P66 (ROP1) | Recombinant proteins with CKS domain | IgM ELISA | 142 total; 89 acute, 53 recent seroconversion |
Sensitivity: 74.6%; specificity, 95.7% | |
| P22 (SAG2), P25 (H4), P29 (GRA7), P35 (GRA8) | Recombinant proteins with MBP domain (P22, P25, P29) and GST domain (P35) | IgG ELISA | 96 total pregnant women; 20 acute, 70 chronic | Sensitivity, 90% (acute-phase), 98.6% (chronic sera); specificity, 97% (acute- phase) |
Li et al., 2000b |
| SAG1, GRA1, GRA7 | Recombinant proteins with His tag domain | IgG ELISA | 241 total, 117 acute, 124 chronic |
Sensitivity, 100% acute-phase sera, 91.1%, chronic sera | Pietkiewicz et al., 2004 |
| MICex2, MAG1, MIC3, | Recombinant proteins with His tag domain | IgG ELISA | 72 chronic sera | Sensitivity, 88.9%; specificity, 100% | Holec et al., 2008 |
| P35 (GRA8), SAG2, GRA6 | Sensitivity, 94.4%; specificity, 100% | ||||
| MAG1, SAG1, GRA5 | Recombinant proteins with His tag domain | IgG ELISA | 189 total; 27 acute, 18 post-acute, 144 chronic | Sensitivity, 92.6%; specificity, 100% | Holec-Gąsior and Kur, 2010 |
| GRA2, SAG1, GRA5 | Sensitivity, 93.1%; specificity, 100% | ||||
| ROP1, SAG1, GRA5 | Sensitivity, 94.2%; specificity, 100% |
a
All recombinant proteins were produced in E. coli unless specified.