TABLE 1.
Initial medium compositions evaluated for rescuing cassava embryos.
| Components |
Medium |
|||||
| M6a | 1/2 MSb | 1/2 NLNc | MSREb | MSRE-Vb | MS2b | |
| Macro- and micro-elements (mg/L) | ||||||
| Ca(NO3)2 × 4H2O | – | – | 360 | – | – | – |
| CaCl2 × 2H2O | 220 | 220 | – | 220 | 220 | 440 |
| NH4 NO3 | 825 | 825 | – | 825 | 825 | 1,650 |
| KNO3 | 950 | 950 | 62.5 | 950 | 950 | 1,900 |
| MgSO4 × 7H2O | 185 | 185 | 62.5 | 185 | 185 | 370 |
| KH2PO4 | 85 | 85 | 62.5 | 85 | 85 | 170 |
| H3BO3 | 3 | 3 | 5 | 3 | 3 | 6 |
| MnSO4 × H2O | 8 | 8 | 9.5 | 8 | 8 | 17 |
| ZnSO4 × 7H2O | 4.3 | 4.3 | 5 | 4.3 | 4.3 | 8.6 |
| Na2MoO4 × 2H2O | 0.13 | 0.13 | 0.125 | 0.13 | 0.13 | 0.25 |
| CuSO4 × 5H2O | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 |
| CoCl2 × 6H2O | 0.025 | 0.025 | 0.0125 | 0.025 | 0.025 | 0.025 |
| KI | 0.42 | 0.42 | – | 0.42 | 0.42 | 0.83 |
| Na2-EDTA | 18.7 | 18.7 | – | 18.7 | 18.7 | 37.3 |
| FeSO4 × 7H2O | 13.9 | 13.9 | – | 13.9 | 13.9 | 27.8 |
| Vitamins, amino acids, other organics supplements (mg/L) | ||||||
| Nicotinic acid | – | 0.25 | 2.5 | 0.25 | 0.5 | 0.5 |
| Pyridoxine | – | 0.25 | 0.25 | 0.5 | 0.5 | |
| Thiamine | – | 0.05 | 0.25 | 0.05 | 0.1 | 0.1 |
| Glycine | – | 1 | 1 | 1 | 2 | 2 |
| Myo-inositol | – | 50 | 50 | 50 | 100 | 100 |
| Biotin | – | – | 0.025 | – | – | – |
| L-glutamine | – | – | 400 | – | – | – |
| Folic acid | – | – | 0.25 | – | – | – |
| Glutathione | – | – | 15 | – | – | – |
| L-serine | – | – | 50 | – | – | – |
| 2,4 D | – | – | – | – | – | 2 |
| NAA | – | – | – | 0.01 | 0.01 | – |
| Kinetin | – | – | – | – | – | 0.5 |
| GA3 | 1 | – | – | 1 | 1 | 1 |
| Sucrose | 30,000 | 1,30,000 | 1,30,000 | 30,000 | 30,000 | 80,000 |
| Gellan Gum | 3,000 | 3,000 | 3,000 | 3,000 | 3,000 | 3,000 |