FIGURE 6.

Pathogenicity and plant infection assays of ChCDC25 knockout mutants on Arabidopsis leaves. (A) A 10 μL droplet of conidial suspension (1 × 10⁵ conidia/mL) placed each wounded Arabidopsis leaf and then incubated at 25°C for 4 days. (B) Infection structures in Arabidopsis leaf cells. Scale bar, 20 μm. Hy, hyphae; Ap, appressorium; Ph, primary hypha; Sh, secondary hypha. (C) Microscopic examination of fungal development in leaf tissues. For each strain, at least 100 conidia were measured for every replicate. Three replicates were examined and three independent experiments were conducted. (D) Pathogenicity assay of the ChCDC25 mutants on wounded leaves. Conidial suspensions (10 μL) were placed on wound sites of Arabidopsis leaves and incubated at 25°C for 3 days.