Figure 1.

Lymphocyte distribution in B16F0 mouse melanoma. (A) Overview image of the tumor and surrounding tissue labeled with H&E staining (left) or multicolor immunofluorescence (right). (B–D) show close-up of rectangles 1, 2, and 3 from panel (A). Green represents CD4⁺ T cells, cyan represents CD8⁺ T cells, red represents B220/CD45R⁺ B cells and blue indicates DAPI-stained nuclei. Yellow dashed curves outline subcutaneous fibrous tissue that constitutes the tumor capsule. Yellow dotted curves outline regions that surround vessel and are enriched in leukocytes. Cyan dotted curves on H&E images show blood vessels and capillaries that have no prominent leukocyte pockets. It should be noted that tissue structures are marked based on H&E images; these marks do not coincide directly with cells in the fluorescence images since these show different slices spaced ~20 μm apart.