Figure 3.

Identification of CD8⁺ T cell clones heterogeneously distributed within tumor samples from a mouse model of melanoma. (A). The concentration of each TCRβ CDR3 clonotype in the first control sample is plotted against the concentration of the same clonotype in the second control sample. (B). The concentration of each TCRβ CDR3 clonotype in tumor section #3 (y-axis) is plotted against the concentration of the same TCR clonotype in tumor sections #2 and #4 (x-axis). Clonotype variants that were identified as significantly expanded in one of the tumor sections are shown in orange or blue. (C). Numbers of expanded or contracted clones between all pairs of repertoires in four experimental and three control tumor. (D). Pearson's r measurement of correlation between counts of clones present in each pair of control or experimental repertoires.