Table 1.

Cells, molecules and clonotypes in replicates of B16F0-infiltrating T cells.

Setup	Cell subset	# T cells sorted	# TCRβ UMIs	# clonotypes	Clonality*
Experimental	CD4	11,245	3,710	1,417	0.03
Experimental	CD4	6,519	1,737	781	0.03
Experimental	CD4	10,922	6,757	1,967	0.03
Experimental	CD4	8,904	3,015	1,280	0.03
Experimental	CD8	14,270	12,708	1,052	0.24
Experimental	CD8	10,375	12,597	786	0.3
Experimental	CD8	22,436	29,176	1,483	0.31
Experimental	CD8	14,284	17,355	1,030	0.31
Control	CD4	44,232	5,210	2,174	0.03
Control	CD4	25,182	17,836	3,691	0.03
Control	CD4	27,891	10,852	3,004	0.04
Control	CD4	13,022	4,106	1,572	0.04
Control	CD8	20,794	15,361	2,365	0.21
Control	CD8	13,022	3,109	734	0.21
Control	CD8	14,112	13,614	1,800	0.23
Control	CD8	6,494	3,556	693	0.23

^*Clonality metrics [1-Normalized ShannonWiener Index] was calculated as in Tumeh et al. (10) but after down-sampling to an equal number of 500 UMI-labeled TCRβ cDNA molecules (39).