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. 2020 May 8;8:302. doi: 10.3389/fcell.2020.00302

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Copyright © 2020 Chen, Liang, Xi, Yang, Shan, Wang, Zhong, Xu, Yang, Sun, Sun and Bian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nrf2 inhibitor ML385 counteracted BM-MSCs coculture induced antioxidation and GFAP/VIM switch. Astrocytes were administrated with or without ML385 for 6 h, then followed by 30 μM hemin incubation, with or without BM-MSCs coculture treatment. (A) TUNEL staining (red) was used to mark apoptotic cells. Bar = 50 μm. (B) The results of the apoptosis rate and densitometric analysis of the bands were plotted into a histogram of five randomly fields. (C,D) Western blotting analysis of Cx43, GFAP, VIM, Nrf2, HO-1 protein expression was examined. The relative expression was normalized to control. All data are displayed as means ± SD (n = 3). The difference between groups was analyzed using One-way ANOVA test. *p < 0.05, **p < 0.01, ***p < 0.001, compared with control (ML385 0 μM).