FIGURE 7.

Cx43 knockdown suppressed BM-MSCs-induced Nrf2 nuclear translocation. (A,B) Western blotting analysis of Nrf2 expression in control, si-NC, si-Nrf2 transfected astrocytes. (C) Immunofluorescence staining of control, si-NC, si-Cx43 treated astrocytes with anti-Nrf2 (red). The siRNA was labeled with fluorophore FAM (green) to show the transfected cells. The cell nuclei were counterstained with DAPI (blue). Bar = 50 μm. (D) Western blotting analysis of cytoplasmic and nucleus extraction samples from control, si-NC, si-Cx43 transfected astrocytes with anti-Nrf2. GAPDH and H3 were used as a loading control for cytoplasmic and nucleus protein, respectively. (E) The histogram showing the results of densitometric analysis of nucleus/cytoplasmic Nrf2 expression in control, si-NC, si-Cx43 transfected astrocytes. All data are displayed as means ± SD (n = 3). The difference between groups was analyzed using One-way ANOVA test. *p < 0.05, ***p < 0.001.