. 2020 May 15;26(8):1076–1081. doi: 10.1016/j.cmi.2020.05.007

Table 1.

Parameters of commercial quantitative real-time PCR kit used as reference methods

Group	Manufacturer	Gene	RNA (μL)	Temperature and cycles	Result (C_t)		Lowest detection limit (copies/mL)	Internal control
Group	Manufacturer	Gene	RNA (μL)	Temperature and cycles	+	—	Lowest detection limit (copies/mL)	Internal control
A, B	BioGerm Biotechnology Co Ltd	ORF1ab, N	5	60°C, 40 cycles	≤38	>38	1000	Yes
C	Sansure Biotechnology Co Ltd	ORF1ab, N	10/20	60°C, 45 cycles	≤40	>40	500	Yes
D	BGI Co Ltd	ORF1ab	10	60°C, 40 cycles	≤40	>40	100	Yes

Data from manufacturers' instructions. C_t, cycle threshold.

Groups are as follows: A, Jiangsu Center for Disease Control (CDC); B, Zhejiang CDC; C, Datong CDC, Shanxi Province and Fifth People's Hospital of Datong City, Shanxi Province; and D, Hubei CDC.