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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Cell Stem Cell. 2009 May 8;4(5):440–452. doi: 10.1016/j.stem.2009.03.003

Figure 2. Characterization of SSEA-1+ and SSEA-1− Cells In Vitro from Various GBM-Derived TSCs/TICs.

Figure 2

(A) Soft agar colony-forming assay to determine clonogenicity of SSEA-1+ versus SSEA-1− cells from three different GBM lines.

(B) Limiting dilution sphere-forming assay. Three cell lines used in (A) were plated into 96-well plates with various seeding densities (5–50 cells per well, 30 wells per each condition). Data from a representative experiment were shown. R2 > 0.83.

(C and D) PCNA staining (C) and cell-cycle analysis (D) of 1228 and 0131 cells by flow cytometry. Error bars represent SD (performed in triplicates); *p < 0.05.