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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Cell Stem Cell. 2009 May 8;4(5):440–452. doi: 10.1016/j.stem.2009.03.003

Figure 4. Lineage Tracking of SSEA-1+ versus SSEA-1− GBM Cells.

Figure 4

(A) Representative microphotograph of the resultant cells that were cultured for 2 weeks after the mixture of 0905-derived SSEA-1+ (red) and SSEA-1− (green) at the indicated ratios. Cells were harvested at a 2 week time point and prepared for secondary sphere-forming assay (the bottom row) or quantitation by flow cytometry (B). These cells were further stained with SSEA-1-PE antibody, and the representative data from the mixture of 5% SSEA-1+ and 95% SSEA-1− cells are shown (C). Spheres generated after the initial 2 week cultures were counted in each condition (D). Error bars represent SD; *p < 0.05.