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. 2019 Oct 31;16(3):673–683. doi: 10.1080/21645515.2019.1669414

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© 2019 Instituto Butantan. Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — rBCG-LTAK63 increases NO production at 24 h after intraperitoneal immunization.

(a) Analysis of cell population was performed in the peritoneal fluid of BALB/c mice 24 h after intraperitoneal immunization with BCG or rBCG-LTAK63 (1 x 10⁶ CFU). At 24 h after immunization, the production of (b) NO and (c) H₂O₂ was evaluated in the cells isolated from the peritoneum. The production of (d) TNF-α and (e) IFN-γ was evaluated by the CBA inflammatory assay (non-stimulated ex vivo). The control group received Saline. The results represent one of two independent experiments with similar results (n = 4–5 animals per group). Statistical significance was determined by one-way ANOVA. Significant differences were observed for indicated groups (* P < .05; ** P < .01; *** P < .001).