Figure 7.
Kinetics of weight variation and histopathological analysis after challenge.
(a) BALB/c mice immunized with BCG or rBCG-LTAK63 (1 x 106 CFU) 90 d before intratracheal challenge with Mtb H37Rv (1 x 105 CFU) and the kinetics of weight variation was followed. (b) On day 15 after challenge, lungs from BALB/c immunized mice or those receiving Saline were fixed and sections were stained with HE prior to histopathological analysis. Non-challenged mice were used as Control. Images show representative lung sections (100 x magnification; bars scales correspond to 50 µm). Morphometrics of lung sections is shown. The results represent one of two independent experiments with similar results (n = 6–8 animals per group). Bars are mean ± SEM. Statistical significance was determined by one-way ANOVA in (a) and by Student’s t-test in (b).