Table 3.

Mutated sites by numbers of mutations present in cells from autologous tumor cell line.

Sites of mutations	Significance
CTNNB1	β-catenin: coordinates cell-cell adhesion & gene transcription
GATA2	Regulates gene expression critical for embryogenesis & self-renewal
KDR	a vascular endothelial growth factor receptor, VEGFR-2
PRKCB	Protein Kinase C, tumor suppressor of aberrant signal transduction
NFKBIA	Inhibits NFKB which controls transcription, cytokine production, & apoptosis
PIK3CD	Enzymes that enhance cell growth, proliferation, motility & apoptosis
PAK1	P21 activated kinase, regulate cell proliferation, differentiation, motility, apoptosis, and development of dendrites & filopods
KITLG	KIT ligand stem cell factor, binds cKIT (CD117)
ITPR1	Tumor suppressor and induces apoptosis
FLT4	Encodes vascular endothelial growth factor receptors C and D
WNT2	WNT pathway signaling important in embryogenesis & oncogenesis
PGF	Placental growth factor, a member of the VEGF family
DCC	Tumor suppressor (deleted in colon cancer)
PHLPP1	Tumor suppressor (regulates PKC)
ITPR1	Inhibits cell proliferation and induces apoptosis
COL1A1	Type 1 collagen synthesis
COL1A2	Type 1 collagen synthesis

Tumor samples were obtained 4 and 13 months after initial diagnosis. In terms of prevalence of mutations, results were the same for cells from both cell cultures. Mutations are in rank order based on expression of the mutation.