Fig. 2.

Design strategies for EHT system using hiPSC-CMs. (A) Schematic diagram depicting design strategy to produce EHTs. hiPSCs can be differentiated into beating hiPSC-CMs via temporal Wnt signaling modulation. hiPSC-CMs were then seeded into one of three tested scaffolds including degradable PGA, rat tail-derived collagen type I and decellularized porcine ECM with controllable fiber alignment (B, C and D) for the scaffold for engineering the cardiac tissue. For beating PGA, 0.7 million hiPSC-CMs were seeded onto 5 mm × 5 mm squares of PGA and cultured for two weeks. 1.2 million hiPSC-CMs were seeded into the wells in 2% agarose molds for the generation of beating cardiac rings. 1 million cells were seeded onto thin sections of decellularized porcine myocardial extracellular matrix. Scale bars = 100 μm.