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. 2019 Dec 4;16(4):907–918. doi: 10.1080/21645515.2019.1688038

Figure 3.

Figure 3.

Construction and expression of the ZIKV DNA plasmid-vaccinated murine spleen-derived ZIKV dMAb plasmid constructs.

In vivo expression of ZIKV dMAb. CAnN.Cg-Foxn1nu/Crl nude mice aged 6–8 weeks received 100 μg intramuscular injection in the right tibialis anterior muscle followed by CELLECTRA® enhanced electroporation delivery. Serum IgG level was measured at various time points and quantified via ELISA. (a and c) Binding of recombinant ZIKV Env protein (rZIKV-E) with sera from anti-ZIKV dMAb-administered mice. rZIKV-E coated ELISA plates were probed with D 14 sera from ZIKV dMAbs or pVax1-injected mice. The mean OD450mn values are shown ±SD. (b and d) Expression of the ZIKV dMAb clones ID4G7, 8D10F4, 8A9F9, and 3F12E9 by SDS-polyacrylamide gel electrophoresis and Western Blot. The Western blot analysis shows rZIKV-E probed with mouse sera containing dMAbs 14 d post the dMAb plasmid injection, and the murine sera bound to anti-human IgG-IRDye 800-CW conjugated secondary antibodies.