FIG 2.
CMTR1 is required for the antiviral response. (A) Focus-forming assay (FFU) of supernatants. (B) RT-qPCR analysis of viral RNA, relative to GAPDH, harvested from ZIKV- or DENV-infected Huh7 cells (48 h postinfection [hpi]; multiplicity of infection [MOI] of 0.01) treated with the indicated siRNAs. (C) (Left) Representative fields of ZIKV- and DENV-infected Huh7 cells (48 hpi; MOI 0.01) treated with the indicated siRNAs and immunostained with anti-flaviviral E protein (green). Nuclei were stained with DAPI (blue). Scale bar: 100 μm. (Right) Quantification of the percentages of ZIKV- and DENV-infected Huh7 cells. (D) Quantification of the relative numbers of VSV-GFP-positive Huh7 wild-type (WT) and STAT1KO cells after siRNA treatment and IFN-β pretreatment (25 U/ml; 16 h) determined at 8 hpi and set to a value of 100. For panels C and D, ≥5,000 cells were counted in each experiment per condition. Values represent means ± SEM of results from 3 biological replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (unpaired Student's t test). n.s., not significant.