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. 2020 May 13;5(3):e00227-20. doi: 10.1128/mSphere.00227-20

FIG 11.

FIG 11

Characterization of CPAR2_603600-null mutant in CBS1954 strain. (A) Deletion mutant CBS1954 Δ/ΔM20/1 generated in environmental isolate CBS1954 showed similar growth defects under iron-restricted conditions. (B) Expression of CPAR2_603590 and CPAR2_304050 in the absence of CPAR2_603600 (*, P ≤ 0.05). (C) Decrease in filamentous growth on solid Spider medium. (D) Reduction in biofilm formation in the mutant. Statistical significance was calculated using the nonparametric t test (****, P ≤ 0.0001). (E) Alteration of expression of genes involved in iron metabolism in the homozygous deletion mutant. (F) Expression of biofilm-related genes in YPD medium after growth at 30°C (*, P ≤ 0.05; ****, P ≤ 0.0001). (G) Expression of biofilm-related genes in Spider medium after growth at 37°C. Statistical significance was calculated using one-way ANOVA with Dunnett’s post hoc test.