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Loss of CPAR2_603600 results in decreased biofilm formation. The CLIB 214 wild-type, deletion mutant, and reintegrant strains were grown in Spider medium at 37°C for 48 h, and biofilm formation was determined using the XTT cell viability assay. Three independent experiments were performed for biofilm analysis by XTT assay. One-way ANOVA with Dunnett’s post hoc test was used to determine statistical significance (**, P ≤ 0.01; ****, P ≤ 0.0001).
