FIG 8.

Addition of excess iron to the preculture medium partially rescued the biofilm defect of the deletion mutants. Addition of 2 mM FeCl₃ to the YPD preculture medium partially rescued the biofilm formation defect. After preculturing of all strains in iron-supplemented YPD, cells were transferred to liquid Spider medium and incubated at 37°C. After 48 h, the XTT assay was applied to measure potential alterations in biofilm formation. Two independent experiments were performed for the biofilm assay. One-way ANOVA with Dunnett’s post hoc test was used to determine statistical significance (**, P ≤ 0.05).