FIG 9.

Expression of genes involved in iron metabolism in the homozygous deletion mutant. Expression analysis of 14 genes related to iron metabolism (GO term analysis) was performed using qPCR. The expression levels shown represent results of comparisons to the normalized wild-type (WT) values (WT value = 1; marked by line) for each gene examined. The cells were grown in YPD medium at 30°C for 4 h prior to total RNA isolation. Statistical significance was determined using one-way ANOVA coupled with Dunnett’s post hoc test (*, P ≤ 0.05; **, P ≤ 0.01; ****, P ≤ 0.0001).