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. 2020 May 7;14:438. doi: 10.3389/fnins.2020.00438

TABLE 2.

Exosome isolation workflow.

SC EQ Plasma
Exosome isolation 1. Filtered plasma 1 ml.
2. ExoEasy spin column.
3. Elute and collect the EVs.		 1. Filtered plasma 0.5 ml.
2. Thrombin for fibrin treatment.
3. ExoQuickTM solution for precipitation.
4. Resuspend the EVs pellet.		 N/A
miRNA extraction 1. Small RNA extraction following the QIAzol protocol.
2. Small RNA measurement.
Library preparation and sequencing 1. 200 ng of total RNA, MGIEasy Small RNA Library Prep Kit.
2. BGISEQ-500 platform.
3. Acquire > 25M reads per each sample.