TABLE 3.
Characteristics of 23 newly developed polymorphic microsatellite loci in Menispermum.
| Primer sequences (5′-3′) | Repeat motif | Fluorescent dye | Allele size (bp) | T (°C) | NA | Ho | He | PIC* | GenBank accession no. | |
| CPSSR_1 | F: GGTTCTTGAAACGGGCTTGT R: CCGTTGTCTCCCTGTTCCTC |
(AAAAG)5 | FAM | 159–208 | 57 | 3 | 0.157 | 0.514 | 0.424 | MK153148 |
| CPSSR_30 | F: GCGAGAACCACCTCCCTAAC R: AGTGCTGCAATGCTCATCCT |
(AAG)7 | HEX | 119–161 | 55 | 6 | 0.542 | 0.689 | 0.641 | MK153146 |
| CPSSR_91 | F: CTCGCACTTGTCCTGACCTT R: GCAACCGTTGAGACCGTTTC |
(AGACGA)5 | ROX | 118–178 | 57 | 4 | 0.319 | 0.635 | 0.568 | MK153147 |
| CPSSR_95 | F: TCGGTTAGTCCTCCTACGTG R: ATGCTGGAGCTGCTGAAACT |
(AGC)7 | TAMRA | 129–165 | 56 | 7 | 0.922 | 0.6 | 0.524 | MK153142 |
| CPSSR_157 | F: CGCTTTCCGTCACCAGTAGT R: TCTTCACCGATCCTCGCAAG |
(CAA)6 | HEX | 141–183 | 57 | 2 | 0.383 | 0.493 | 0.371 | MK153138 |
| CPSSR_167 | F: AACCACTTCACCACTGCCAT R: TGTTGGGCTAGGAGTTGCTG |
(CAG)5 | ROX | 164–194 | 55 | 3 | 0.369 | 0.593 | 0.502 | MK153145 |
| CPSSR_172 | F: CCACTCCGGTGAAGGAAGAG R: AGTCACCTTTGCCGGATCTG |
(CAGCT)5 | TAMRA | 129–177 | 55 | 8 | 0.947 | 0.729 | 0.69 | MK153140 |
| CPSSR_175 | F: TGGCATGGTGTGGAAGGATC R: ACATGCACTGTGGAGGGAAG |
(CAT)6 | FAM | 138–174 | 57 | 5 | 0.957 | 0.62 | 0.553 | MK153155 |
| CPSSR_177 | F: AAGACGGGCCTCATCAATCG R: TCTGCCAGGTTTTCTGCACT |
(CAT)8 | HEX | 128–176 | 57 | 6 | 0.283 | 0.685 | 0.624* | MK153158 |
| CPSSR_183 | F: CGTTTGATGACGCCGTTTGT R: TCTGAGGGAGGAGGTGACTG |
(CCA)5 | ROX | 105–135 | 57 | 5 | 0.226 | 0.516 | 0.474* | MK153150 |
| CPSSR_194 | F: AGAACTTGCTCTCGCTGGTC R: ACACGGCGAAAGATGTCGAT |
(CCG)7 | TAMRA | 111–153 | 57 | 5 | 0.217 | 0.595 | 0.507 | MK153149 |
| CPSSR_201 | F: CATCCCTTGTCTGCTTCCGT R: TCAATTTCCCTGGCGGAGTC |
(CGC)7 | FAM | 133–175 | 55 | 8 | 0.567 | 0.634 | 0.597 | MK153139 |
| CPSSR_231 | F: GGCCATTGCTGCAGTGTTAC R: TTCCAGAGAAATGCCGGCTT |
(CTA)6 | FAM | 156–192 | 57 | 4 | 0.242 | 0.659 | 0.582* | MK153143 |
| CPSSR_261 | F: GGAGAAGCTCTATCCTCTCCCT R: CTGCGGCTCGGACAAAGTAT |
(CTTCT)7 | TAMRA | 94–165 | 57 | 4 | 0.25 | 0.635 | 0.56* | MK153157 |
| CPSSR_314 | F: GTTCCAAATTGGGCCTCTGC R: CCACTGAGCCTCTCTCTTGC |
(GAT)6 | FAM | 133–169 | 57 | 7 | 0.123 | 0.645 | 0.584* | MK153144 |
| CPSSR_365 | F: ACGAAGAGAAAACCGTCGCT R: CCCTTAAGCCCTACCCTCCT |
(GTG)5 | ROX | 113–144 | 56 | 3 | 0.368 | 0.314 | 0.266 | MK153152 |
| CPSSR_396 | F: CGACGATCTCTGCCTCGAAT R: AGGGTGCTAGGGACTCGAAT |
(TAC)6 | FAM | 154–190 | 57 | 5 | 0.195 | 0.591 | 0.503* | MK153137 |
| CPSSR_459 | F: ACAGGGATTTCACGGCTGAG R: GCCTCCCTTGAACCCTTCTC |
(TCG)6 | HEX | 109–145 | 56 | 4 | 0.179 | 0.195 | 0.181 | MK153154 |
| CPSSR_475 | F: TCCATCACTCGTCTCCTCCT R: GATGCCCTAGACGAGAAGCC |
(TCT)6 | ROX | 131–167 | 57 | 4 | 0.265 | 0.598 | 0.514 | MK153159 |
| CPSSR_502 | F: TCGGTGCTGTCTCTTCTTCC R: TCAGCTTGTCTTCCCCGTTC |
(TTA)6 | TAMRA | 162–198 | 57 | 3 | 0.059 | 0.559 | 0.469* | MK153151 |
| CPSSR_517 | F: GAGACGAAAGGGCAGAGTCC R: GACAGTGGCAGGAAAGTTGC |
(TTC)7 | FAM | 126–168 | 56 | 6 | 0.383 | 0.71 | 0.659* 0.49 |
MK153153 |
Locus name, forward (F) and reverse (R) primer sequences, SSR motifs, size of the original fragment (bp), optimal annealing temperatures (T), the total number of alleles per locus (NA), observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), and GenBank accession numbers are given. *Significantly departures from Hardy–Weinberg equilibrium (P < 0.05).