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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: J Vis Exp. 2019 Aug 6;(150):10.3791/60145. doi: 10.3791/60145

Figure 3: Representative qPCR analysis of unc-17-neuron specific markers (tph-1, unc-47 and snb-1) in whole-animal versus unc-17::GFP reporter cell isolates confirming specific enrichment of cholinergic neurons of interest.

Figure 3:

Additional negative controls used here were myo-3::GFP reporter-expressing muscle cells, which had been isolated following the same protocol. Data show mean values ± SD (n = 3 biological replicates). *p < 0.05; **p < 0.01; ***p < 0.001 by paired t test. This figure has been modified from Germany et al.12.