Figure 4.

Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680) and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum, rat L6 skeletal muscle myotubes were stimulated for the indicated time from 1 hour to 24 hours and interleukin-6 mRNA levels, relative to TATA-BOX, was measured by quantitative real time polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (100 nM and 10 μM), PSB 603 (10 μM), CGS21680 (100 nM) and forskolin (1 μM) (a) Stimulation was performed up to 1 hour (b) Stimulation was performed for up to 3 hours (c) Stimulation was performed for up to 24 hours, data were represented as means ± standard error of mean of three independent experimental group (n=3; *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001), data were analyzed using one way ANOVA test followed by Tukey test