KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals | ||
| Hygromycin | Research Products International Corp. | Cat. NO.: H75000 |
| G418 | Research Products International Corp. | Cat. NO.: G64000 |
| Nourseothricin | Jena Bioscience | Cat. NO.: AB-102–25G |
| Zeocin | VWR | Cat. NO.: AAJ67140–8EQ |
| Propidium iodide | Sigma- Aldrich | Cat. NO.: P4864–10ML |
| Experimental Models: Organisms/Strains | ||
| Mouse: A/Jcr | The Jackson Laboratory | CRL:563 |
|
Cryptococcus neoformans strain XL280α (MFα, mild type serotype D strain) |
[5] | Strain ID: XL280α |
|
Cryptococcus neoformans strain H99 (MFα, wild type serotype A strain) |
[23] | Strain ID: H99 |
|
Cryptococcus neoformans strain KN99 (MFa, wild type serotype A strain) |
[56] | Strain ID: KN99 |
|
Cryptococcus neoformans strain YZ83 (MFα, DMC1::NAT serotype A) |
This manuscript | Strain ID: YZ83 |
|
Cryptococcus neoformans strain YZ463 (MFa, DMC1::NAT serotype A) |
This manuscript | Strain ID: YZ463 |
|
Cryptococcus neoformans strain YZ478 (MFα, REC8::NAT serotype A) |
This manuscript | Strain ID: YZ478 |
|
Cryptococcus neoformans strain YZ486 (MFa, REC8::NAT serotype A) |
This manuscript | Strain ID: YZ486 |
|
Cryptococcus neoformans strain YZ175 (MFα, PH2B-H2B-GFP-HYG serotype A) |
This manuscript | Strain ID: YZ175 |
|
Cryptococcus neoformans strain YZ187 (MFα, DMC1::NAT, PH2B-H2B-GFP-HYG serotype A) |
This manuscript | Strain ID: YZ187 |
|
Cryptococcus neoformans strain YZ63 (MFα, FRDMC1, Serotype A) |
This manuscript | Strain ID: YZ63 |
|
Cryptococcus neoformans strain YZ659 (MFα, FRREC8, Serotype A) |
This manuscript | Strain ID: YZ659 |
|
Cryptococcus neoformans strain YZ661 (MFα, FRREC8, Serotype A) |
This manuscript | Strain ID: YZ661 |
| Oligonucleotides | ||
| See Table S3 | This manuscript | N/A |
| Recombinant DNA | ||
| pYZ-FRDMC1 (pXL-PDMC1-CRE-PTEF1-NAT-NEO) |
This manuscript | Plasmid ID: pYZ-FRDMC1 |
| pYZ-FRREC8 (pXL-PREC8-CRE-PREC8-NAT-NEO) |
This manuscript | Plasmid ID: pYZ-FRREC8 |
| pYZ99 (pXL1-PH2B-H2B-GFP-HYG) |
This manuscript | Plasmid ID: pYZ99 |
| pYZ179 (pXL1-PTUB4-TUB4--tdTomato-NEO) |
This manuscript | Plasmid ID: pYZ179 |
| Software and Algorithms | ||
| R 3.6 | The R Foundation | https://www.rproject.org/ |
| ggplot2 | [48] | https://ggplot2.tidyverse.org/ |
| Colonyzer | [49] | https://research.ncl.ac.uk/colonyzer/ |