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(A) CRISPR-mediated EGFR knockout and homology directed repair (HDR). (B) Agarose gel electrophoresis of PCR-based genotyping for identification of EGFR knockout clones. (C) Western blot analysis of RC21 EGFRwt/wt and EGFR-/- cells. (D) Flow cytometric analysis shows EGFR expression in RC21 EGFRwt/wt and EGFR-/- cells.
