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. 2020 May 15;15(5):e0233163. doi: 10.1371/journal.pone.0233163

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© 2020 Ceyhan et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — A) Increased apoptosis in Inpp4b^-/- testes of 3-month old males on LFD and HFD mice. Cell apoptosis was analyzed by TUNEL assay. A representative image from each group is shown. Scale bar represents 200 μm. B) Apoptotic cells (brown) were counted per field under 20X objective in at least 5 fields in each of 2 sections per animal. *p<0.05; n = 3/group. Data shown as mean ± SEM. Statistical analysis was performed using 2-way ANOVA. C) Magnified sections from Inpp4b^-/- males on HFD. Spermatogonia and cells in meiotic metaphase showed by arrowheads and arrows respectively. Scale bar is 20 μm.