Figure 2.

Weights of pregnant dams and offspring exposed to filtered air (FA), e-cigarette aerosol (PG/VG), or e-cigarette aerosol with nicotine. (A) Pregnant dams were weighed daily throughout gestation ($+\text{Nic}$). Offspring were weighed on (B) PND21 and again in (C) adulthood at the completion of behavioral testing, and body mass (in grams) were compared across treatment groups. For maternal weight, plots are mean $\pm \text{SE}$ and trend lines represent estimated growth curves using linear mixed-effects modeling with treatment and day as fixed effects and dam as random effects. For offspring weights, *$p<0.05$, **$p<0.01$ as determined by linear mixed-effects modeling with treatment and sex as fixed effects and litters as random effects. Plots represent individual mice; bars represent marginal means $\pm \text{SE}$. FA (10 litters), males [$n=55$ (PND21) and $n=23$ (adulthood)], females [$n=41$ (PND21) and $n=23$ (adulthood)]; PG/VG (9 litters) [males $n=41$ (PND21) and $n=20$ (adulthood)], females [$n=45$ (PND21) and $n=23$ (adulthood)]; $+\text{Nic}$ (8 litters) males [$n=33$ (PND21) and $n=19$ (adulthood)], females [$n=45$ (PND21) and $n=18$ (adulthood)]. Note: PG/VG, propylene glycol/vegetable glycerin; PND, postnatal day; SE, standard error; $+\text{Nic}$, propylene glycol/vegetable glycerin plus nicotine.