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. 2020 May 15;15(5):e0230354. doi: 10.1371/journal.pone.0230354

Fig 4. Expression of (A) SULF1 and (B) HSPG2 mRNA by human bone marrow fibroblasts stimulated by CM of M2-Mϕs.

Fig 4

HS27A and primary bone marrow stromal cells (BMSC) were treated with fresh CM from M2-Mϕs for 48h and RNA was subsequently isolated for qPCR. The expression of SULF1 and HSPG2 mRNA was normalized to that of GAPDH. Values obtained for untreated cells (black columns) were arbitrarily set to 1 for comparison. Data shown represent the mean ±SD of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.