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. 2020 Apr 9;128(4):047005. doi: 10.1289/EHP5843

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EHP is an open-access journal published with support from the National Institute of Environmental Health Sciences, National Institutes of Health. All content is public domain unless otherwise noted.

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Figure 1 comprises studies 1, 2, and 3. Study 1 has the following chemical compounds: ADONA, PFESA1, PFHxA, PFHxS, PFOA, PFOS, and GenX Free Acid solubilized in 0.4 percent DMSO and assessed in the DevTox Assay, DNT Assay, and for analytical chemistry. Study 2 has GenX Free Acid chemical compound solubilized in DI water and assessed in the DevTox Assay, DNT Assay, and for analytical chemistry. Study 3 has the following chemical compounds: PFBS, PFPeS, PFHxS, PFHpS, and PFOS solubilized in 0.4 percent DMSO and assessed in the DevTox Assay and DNT Assay. — Study design. Zebrafish were semi-statically exposed to test PFAS daily, from $0 - 5$ dpf. At 6 dpf. developmental toxicity, developmental neurotoxicity, and PFAS tissue concentrations were assessed. Test PFAS included in Study 1, solubilized in DMSO (final concentration 0.4% DMSO), are highlighted in light blue. Because GenX Free Acid was not stable in DMSO, the compound was retested in all three assays using DI water as a diluent in Study 2 (highlighted in blue). In Study 3, a set of sulfonic acid aliphatic PFAS solubilized in DMSO were tested in the DevTox and DNT assays (shown in green). Note: ADONA, 4,8-dioxa-3H-perfluorononanoate; DevTox, developmental toxicity; DI, deionized; DMSO, dimethyl sulfoxide; DNT, developmental neurotoxicity; dpf, days post fertilization; GenX Free Acid, perfluoro-2-propoxypropanoic acid; PFAS, per- and polyfluoroalkyl substances; PFBS, perfluorobutanesulfonic acid; PFESA1, perfluoro-3,6-dioxa-4-methyl-7-octene-1-sulfonic acid; PFHpS, perfluoroheptanesulfonic acid; PFHxA, perfluorohexanoic acid; PFHxS, perfluorohexanesulfonic acid; PFOA, perfluoro-n-octanoic acid; PFOS, perfluorooctanesulfonic acid; PFPeS, perfluoropentanesulfonic acid.