. 2020 Apr 9;128(4):047005. doi: 10.1289/EHP5843

Table 2.

Study-specific metrics.

Study		Name	Diluent and/or vehicle	Assay	Concentrations tested ( $μ M$ )	Exposure replicate^a (n)	Control replicate^a (n)	96-well plates chemicals tested across^b (n)
1		ADONA	DMSO	DevTox	0.04, 0.1, 0.4, 1.1, 3.1, 9.3, 27.2, 80.0	6	216	6
				DNT	4.4, 7.9, 14.0, 25.1, 44.8, 80.0	24	394	17
				Chemistry	25.1, 44.8, 80.0	5	5	3
1	GenX Free Acid		DMSO	DevTox, DNT, Chemistry	Not stable in DMSO; results not reported; see Study 2
1		PFESA1	DMSO	DevTox	0.04, 0.1, 0.4, 1.1, 3.1, 9.3, 27.2, 80.0	6	44	1
				DNT	4.4, 7.9, 14.0, 25.1, 44.8, 80.0	24	339	7
				Chemistry	25.1, 44.8, 80.0	4	5	3
1		PFHxA	DMSO	DevTox	0.04, 0.1, 0.4, 1.1, 3.1, 9.3, 27.2, 80.0	6	216	6
				DNT	4.4, 7.9, 14.0, 25.1, 44.8, 80.0	24	394	17
				Chemistry	25.1, 44.8, 80.0	4	4	2
1		PFHxS	DMSO	DevTox	0.04, 0.1, 0.4, 1.1, 3.1, 9.3, 27.2, 80.0	6	216	6
				DNT	4.4, 7.9, 14.0, 25.1, 44.8, 80.0	24	394	17
				Chemistry	14.0, 25.1, 44.8	4	4	2
1		PFOA	DMSO	DevTox	0.04, 0.1, 0.4, 1.1, 3.1, 9.3, 27.2, 80.0	6	216	6
				DNT	4.4, 7.9, 14.0, 25.1, 44.8, 80.0	24	394	17
				Chemistry	25.1, 44.8, 80.0	4	4	2
1		PFOS	DMSO	DevTox	0.04, 0.1, 0.4, 1.1, 3.1, 9.3, 27.2, 80.0	6	216	6
				DNT	0.2, 0.3, 0.6, 1.0, 1.8, 3.1	24	394	17
				Chemistry	1.0, 1.8, 3.1	4	4	2
2		GenX Free Acid	DI water	DevTox	0.04, 0.1, 0.4, 1.1, 3.1, 9.3, 27.2, 80.0	6	44	1
				DNT	4.4, 7.9, 14.0, 25.1, 44.8, 80.0	24	161	4
				Chemistry	25.1, 44.8, 80.0	4	4	2
3		PFBS	DMSO	DevTox	1.7, 3.1, 5.5, 9.8, 17.6, 31.4, 56.0, 100.0	10	140	6
3		PFBS	DMSO	DNT	5.5, 9.8, 17.6, 31.4, 56.0, 100.0	25	327	10
3		PFPeS	DMSO	DevTox	1.7, 3.1, 5.5, 9.8, 17.6, 31.4, 56.0, 100.0	6	44	1
3		PFPeS	DMSO	DNT	3.1, 5.5, 9.8, 17.6, 31.4, 56.0	24	186	4
3		PFHxS	DMSO	DevTox	1.7, 3.1, 5.5, 9.8, 17.6, 31.4, 56.0, 100.0	10	140	6
3		PFHxS	DMSO	DNT	3.1, 5.5, 9.8, 17.6, 31.4, 56.0	25	327	10
3		PFHpS	DMSO	DevTox	1.7, 3.1, 5.5, 9.8, 17.6, 31.4, 56.0, 100.0	10	140	6
3		PFHpS	DMSO	DNT	1.7, 3.1, 5.5, 9.8, 17.6, 31.4	25	327	10
3		PFOS	DMSO	DevTox	1.7, 3.1, 5.5, 9.8, 17.6, 31.4, 56.0, 100.0	10	140	6
3		PFOS	DMSO	DNT	0.5, 1.0, 1.7, 3.1, 5.5, 9.8	25	327	10

Note: ADONA, 4,8-dioxa-3H-perfluorononanoate; DevTox, developmental toxicity; DI, deionized; DMSO, dimethyl sulfoxide; DNT, developmental neurotoxicity; GenX Free Acid, perfluoro-2-propoxypropanoic acid; PFBS, perfluorobutanesulfonic acid; PFESA1, perfluoro-3,6-dioxa-4-methyl-7-octene-1-sulfonic acid; PFHpS, perfluoroheptanesulfonic acid; PFHxA, perfluorohexanoic acid; PFHxS, perfluorohexanesulfonic acid; PFOA, perfluoro-n-octanoic acid; PFOS, perfluorooctanesulfonic acid; PFPeS, perfluoropentanesulfonic acid.

^{^a}

Replicate numbers indicate single animals except for chemistry samples comprising pools of 10 larvae.

^{^b}

Indicates total number of 96-well microtiter plates assessed for each study and/or assay. For Study 1 DevTox and DNT assays, ADONA, GenX Free Acid, PFESA1, PFHxA, PFHxS, PFOA, and PFOS were tested in parallel and shared the same 0.4% DMSO control samples. PFESA1 was obtained subsequently and had unique, experiment-specific control data. In Study 1, GenX Free Acid diluted in 0.4% DMSO was determined to be unstable, resulting in a null data set. In Study 2 (Figure 1), zebrafish were exposed to GenX Free Acid diluted in DI water and evaluated in the DevTox and DNT assays. Measured media and tissue doses were also obtained. Study 3 (Figure 1) examined the ability of PFBS (4-carbon), PFPeS (5-carbon), PFHxS (6-carbon), PFHpS (7-carbon), or PFOS (8-carbon) exposure to cause developmental toxicity or developmental neurotoxicity. All chemicals tested in Study 3, except PFPeS, were exposed in parallel and have shared DMSO control data. PFPeS was synthesized for this study and tested separately, with an experiment-specific DMSO control.