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. 2020 Apr 30;128(4):047011. doi: 10.1289/EHP6262

Figure 3.

Figure 3A is a heatmap titled Erythrocyte development, plotting BcI6, KIf2, Tmod3, Trim58, Rhag, Tal1, Gata1, Klf1, Ankle1, Slc11a2, Epb42, Ank1, Fam210b, Dmtn, and Rhd (right y-axis) across KP1, KP3, KP5, KD5, KD3, KD4, KP2, KD2, KD1, and KP5 (x-axis). Figure 3C is a heatmap titled Heme Metabolic Process, plotting Alas2, Slc25a39, Blvra, Fech, Iba57, Urod, Hmbs, Ppox, Alad, Blvrb, Cpox, Atpif1, Hebp1, Slc11a2, and Uros (right y-axis) across KP3, KP1, KP4, KD3, KD4, KD5, KP2, KD2, KD1, and KP5 (x-axis). Figure 3B and 3D are graphs titled KLF1 and BLVRB, plotting relative mRNA expression, ranging from 0 to 5.0 with increments of 1.0 and 0 to 2.5 with increments of 0.5 (y-axis), respectively, across DMSO, PCB-153, DMSO, and PCB-153 (x-axis) for WT on the left and SXRKO on the right.

Differentially expressed genes in erythrocyte development and heme metabolism in the spleen between steroid and xenobiotic receptor knockout (SXRKO) mice perinatally exposed to polychlorinated biphenyl 153 (PCB-153) and vehicle. (A) Differentially expressed genes (DEGs) involved in erythrocyte development in the spleen of SXRKO mice exposed to PCB-153 compared to SXRKO mice exposed to dimethylsulfoxide (DMSO). (B) Kruppel-like factor 1 (Klf1) erythroid transcription factor quantitative real-time polymerase chain reaction (qPCR) gene expression analysis (n=5pergroup). (C) Heatmap of DEGs related to heme metabolism in the spleen of SXRKO mice exposed to PCB-153 compared to SXRKO mice exposed to DMSO. (D) Biliverdin B (Blvrb) qPCR gene expression analysis (n=5pergroup) in the spleens of WT and SXRKO mice exposed to DMSO or PCB-153. qPCR data plotted as mean fold change over WT DMSO±standarderrorofthemean(SEM). a, statistically significant compared to WT DMSO; b, statistically significant compared to WT PCB; c, statistically significant compared to SXRKO DMSO determined by two-way analysis of variance (ANOVA) and Tukey’s multiple-comparisons test. Note: D, DMSO exposure; K, SXRKO mice; P, PCB-153 exposure; W, WT mice.