Figure 4.

Oxidative stress markers in spleens and red blood cells (RBCs) of 4-wk-old wild-type (WT) and steroid and xenobiotic receptor knockout (SXRKO) mice perinatally exposed to polychlorinated biphenyl 153 (PCB-153) or vehicle. (A) Peroxiredoxin 2 (Prdx2) mRNA expression in the spleens of WT and SXRKO mice exposed to PCB-153 or DMSO, plotted as mean fold change over WT $\text{dimethylsulfoxide}\left(\text{DMSO}\right)\pm \text{standard}\text{error}\text{of}\text{the}\text{mean}(\text{SEM})$ ($n=5\text{per}\text{group}$). (B) Malonaldehyde (MDA) levels of RBC membranes from PCB-153- or DMSO-exposed WT and SXRKO mice ($n=5\text{per}\text{group}$); data plotted as $\text{mean}\pm \text{SEM}$. (C) Heatmap of differentially expressed genes (DEGs) in the spleens between SXRKO mice exposed to PCB-153 ($n=5$) compared to DMSO ($n=5$) for genes related glutathione metabolism. (D) Ratio of reduced glutathione (GSH) to total glutathione in RBCs of WT and SXRKO mice exposed to DMSO or PCB-153; plotted as $\text{mean}\pm \text{SEM}$ ($n=5\text{per}\text{group}$). a, statistically significant compared to WT DMSO; c, statistically significant compared to SXRKO DMSO determined by two-way analysis of variance (ANOVA) and Tukey’s multiple-comparisons test. Note: D, DMSO exposure; K, SXRKO mice; P, PCB-153 exposure; W, WT mice.