Figure 1.

Schematic diagram of N‐linked glycan structure in a mammalian cell, Saccharomyces cerevisiae and, Pichia pastoris. (a) N‐linked glycan structure in mammalian cells commonly generates complex terminally sialylated structures. (b) In S. cerevisiae, the N‐linked glycan structure is typically hypermannosylated (Man > 50GlcNAc2). Moreover, S. cerevisiae core oligosaccharides have terminal α‐1,3 glycan linkages. (c) N‐linked glycan structure in P. pastoris typically is of the Man8‐14GlcNAc2 type with a triantennary‐branched structure. Unlike S. cerevisiae, P. pastoris does not contain potentially immunogenic terminal α‐1,3‐linked mannoses. (d) In Pichia GlycoSwitch® strains (SuperMan5) N‐linked glycan structure is typically hypomannosylated (with a mannose‐5 structure)