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Crossover recombination phenomenon in the Pichia pastoris genome. Following the electroporation process of competent yeast cells, cloned linear vectors are inserted into the electroporated cells. Crossover recombination occurs between 5′ promoter (5′ PAOX1) of vector and AOX1 region of P. pastoris genome. Consequently, cloned cells with a recombinant genome are formed. AOX1, alcohol oxidase 1; TT, transcription termination region
