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. 2019 Aug 14;32(2):129–140. doi: 10.1002/pca.2882

TABLE 3.

Regression equation, limit of detection (LOD), limit of quantitation (LOQ), intra‐ and inter‐day precision, repeatability, stability, recovery and matrix effect of 50 compounds

Name Regression equation r Linear range (ng/mL) LOD (ng/mL) LOQ (ng/mL) Precision Repeatability Stability Recovery Matrix effect
Intra‐day (RSD%; n = 6) Inter‐day (RSD%; n = 3) (RSD %; n = 6) (RSD%; n = 6) Mean RSD%
Chlorogenic acid y = 845x + 4.77e5 0.9999 0.604–755000 0.195 0.585 1.78 1.79 1.55 3.33 99.70 0.23 1.01
Neochlorogenic acid y = 115x + 1.13e5 0.9992 3.01–376000 0.388 1.163 1.83 1.71 0.88 1.61 96.70 3.97 1.00
Cryptochlorogenic acid y = 878x + 6.7e3 0.9993 0.316–79000 0.090 0.271 4.04 4.31 2.42 2.47 100.66 1.62 1.04
3,5‐O‐Dicaffeoylquinic acid y = 1.02e3 x + 1.41e5 0.9996 77.6–388000 14.483 43.449 2.63 2.79 0.72 1.62 98.70 2.65 0.98
3,4‐O‐Dicaffeoylquinic acid y = 602x + 1.45e5 0.9995 7.63–38200 2.094 6.282 3.95 4.39 3.14 4.94 100.67 3.65 1.02
4,5‐O‐Dicaffeoylquinic acid y = 483x + 2.1e4 0.9998 45–28100 7.918 23.755 3.27 3.64 1.16 1.62 98.40 2.12 1.01
1,3‐O‐Dicaffeoylquinic acid y = 5.78e3 x + 5.62e4 0.9996 42–2630 6.971 20.913 3.44 3.28 2.82 3.16 100.92 3.43 1.04
Caffeic acid y = 2.49e3 x + 1.69e5 0.9998 5.76–7200 1.169 3.506 2.86 3.17 3.29 4.83 101.17 2.05 1.03
Quinic acid y = 1.19e3 x + 3.05e5 0.9996 19.6–245000 5.131 15.392 2.73 2.38 1.69 4.18 100.20 2.48 1.05
Protocatechuic acid y = 6.13e3 x + 1.95e5 0.9994 7.68–960 0.780 2.341 2.50 2.30 4.09 3.85 99.50 2.82 0.96
Ferulic acid y = 121x + 3.68e3 0.999 11.2–1400 2.814 8.441 4.21 3.96 3.69 3.58 102.08 4.88 0.99
4,5‐O‐Dicaffeoylquinic acid methyl ester y = 9.67e3 x – 4.3e4 0.9996 5.6–700 1.319 3.958 3.79 4.10 1.78 1.78 98.20 3.48 0.97
Rutin y = 2.5e3 x + 3.14e5 0.9998 0.979–12200 0.036 0.108 2.91 3.21 3.31 3.46 95.90 3.99 1.03
Hyperoside y = 4.32e3 x + 1.77e5 0.9995 0.265–3313 0.058 0.174 1.69 1.55 3.21 3.22 100.32 3.82 1.03
Luteoloside y = 1.07e3 x + 1.34e5 1 0.664–41500 0.056 0.169 1.03 0.92 4.64 3.78 99.16 2.95 1.01
Luteolin y = 100x + 2.47e3 0.9995 124–15600 10.150 30.450 2.28 2.22 1.34 4.09 100.29 4.35 1.00
Rhoifolin y = 1.08e4 x + 572 0.9999 1.3–162 0.280 0.840 2.53 2.75 5.20 4.64 99.82 3.14 0.97
Diosmetin y = 1.15e4 x – 9.93e3 0.9993 1.72–214 0.146 0.438 1.09 0.78 4.63 2.31 100.93 2.95 0.99
Apigenin y = 2.17e4 x – 9.13e4 0.9995 25.4–318 4.188 12.563 3.54 3.83 3.39 3.47 103.09 3.98 0.98
Kaempferol y = 191x + 250 0.9997 2.02–101 0.565 1.695 4.28 4.68 3.59 3.32 101.27 3.01 0.95
Astragalin y = 2.17e4 x + 8.73e4 0.9999 0.0353–883 0.005 0.015 3.51 3.92 3.09 3.51 100.17 3.57 0.97
Lonicerin y = 1.11e3 x + 4.25e3 0.9994 22.4–14000 0.024 0.073 3.45 3.85 2.95 2.74 100.74 2.80 1.01
Kaempferol‐3‐O‐rutinoside y = 3.13e3 x + 9.11e4 0.9999 0.632–7900 0.039 0.117 2.31 2.44 3.79 3.36 99.82 3.18 0.98
Isoquercitrin y = 4.33e3 x + 1.4e5 0.9996 2.65–3310 0.195 0.584 1.68 1.54 4.97 2.91 99.68 2.12 1.02
Sweroside y = 34.9x + 1.56e3 0.9996 0.841–52600 0.231 0.692 3.54 3.68 3.97 4.99 101.78 2.92 1.00
Secologanic acid y = 609x + 3.48e4 0.9999 1.45–182000 0.072 0.217 3.02 3.37 4.87 2.19 99.32 4.05 1.03
Loganin y = 3.95x + 1.72e3 0.999 14.1–35200 4.179 12.536 2.26 2.43 1.83 1.18 103.36 3.89 0.99
Secoxyloganin y = 638x + 1.32e5 0.999 0.189–47400 0.052 0.157 4.19 4.67 2.47 4.68 98.42 3.14 1.02
Loganin acid y = 1.11e3 x – 1.98e5 0.9993 441.2–22100 139.266 417.797 2.95 2.54 4.60 4.55 100.32 2.60 1.03
Morroniside y = 10.4x + 8.21e3 0.999 41.1–514000 7.867 23.600 2.11 1.83 4.32 3.03 101.75 4.18 0.98
Macranthoidin A y = 193x + 9.76e3 0.9997 18.1–22600 2.989 8.967 2.72 1.94 2.43 2.70 103.97 3.66 1.01
Dipsacoside B y = 8.94x + 336 0.9999 3.34–20900 0.745 2.236 2.25 2.37 3.18 3.84 99.81 0.92 1.01
Akebia saponin D y = 6.02x + 8.06e3 0.9996 16.6–207000 1.539 4.618 3.10 1.65 1.46 1.05 102.90 4.10 0.94
l‐Alanine y = 1.43e3 x + 1.78e4 0.9997 8.47–10600 2.228 6.684 3.07 2.60 4.50 3.43 101.09 4.66 1.03
l‐Serine y = 351x + 5.93e4 0.9997 9.16–11500 1.342 4.027 2.71 2.54 3.31 3.40 99.84 1.96 1.02
l‐Proline y = 1.77e3 x + 3.04e4 0.9996 0.381–23800 0.093 0.279 2.75 2.86 2.84 1.13 102.36 2.19 1.01
l‐Valine y = 6.36e3 x + 2.17e5 0.9995 2.51–1570 0.215 0.644 2.96 3.19 1.49 3.33 98.52 3.85 1.04
l‐Threonine y = 1.75e3 x – 9.1e4 0.9996 111–13900 22.325 66.975 2.83 1.46 3.20 2.83 96.47 4.28 0.97
l‐Isoleucine y = 5.15e3 x + 1.92e5 0.9998 2.88–3600 0.422 1.265 0.67 0.63 1.27 2.54 99.49 2.45 1.00
l‐Leucine y = 9.64e3 x + 4.72e5 0.9997 3.38–4230 0.711 2.134 1.21 1.28 4.23 3.05 103.97 2.36 1.01
l‐Aspartic acid y = 660x + 2.38e4 0.9991 4.6–57500 0.835 2.506 1.49 1.66 4.18 4.27 99.27 2.96 0.96
l‐Glutamate y = 1.64e3 x – 2.82e5 0.9998 474–29600 136.177 408.530 0.69 0.74 1.71 1.53 96.93 3.96 1.04
l‐Lysine y = 2.32e3 x – 2.01e5 0.9993 147–12600 36.781 110.342 0.58 0.65 2.15 2.20 95.67 3.73 0.99
l‐Histidine y = 7.11e3 x + 1.65e5 0.9999 59.1–7390 7.656 22.968 1.67 1.45 3.55 3.62 104.62 4.79 1.05
l‐Phenylalanine y = 1.7e4 x + 2.55e5 0.9997 25.6–3200 0.550 1.650 1.78 1.28 3.16 2.36 97.56 3.06 1.03
l‐Arginine y = 6.54e3 x + 1.21e5 0.9996 0.241–1510 0.043 0.128 1.25 1.19 4.54 4.50 100.17 1.98 1.00
Cytidine y = 4.72e4 x – 1.94e4 0.9995 1.84–230 0.496 1.488 3.39 3.20 4.69 3.99 94.20 3.90 1.01
Uridine y = 861x + 7.0e3 0.9997 40.4–5050 7.124 21.372 3.20 3.53 4.19 3.26 100.95 2.55 1.01
Adenosine y = 5.28e4 x + 3.05e5 0.9994 6.31–789 0.945 2.834 1.45 1.41 1.89 3.29 97.76 3.70 0.98
Inosine y = 7.98e3 x + 2.12e4 0.9991 8.78–220 1.847 5.542 1.73 1.87 3.95 3.93 101.52 4.10 0.95