Table 1.
List of antibodies, dilutions, method of antigen retrieval and source manufactures of the immunohistochemical assays
| Antibody (clone) | Antigen retrieval | Dilution | Source |
|---|---|---|---|
| BoHV‐1 (MAb gC‐gIII) | Citrate buffer (pH 6.0) | 1:700 | VRMD (Pullman, WA, USA) |
| BPIV‐3 | EDTA buffer (pH 9.0) | 1:40 | Gently ceded by Dr. Eduardo F. Flores, UFSM |
| BRSV (15c7) | Citrate buffer (pH 6.0) | 1:300 | Gently ceded by Dr. Eduardo F. Flores, UFSM |
| BVDV (15c5) | Citrate buffer (pH 6.0) | 1:1,500 | Gently ceded by Dr. Eduardo F. Flores, UFSM |
| Mycoplasma bovis | Citrate buffer (pH 6.0) | 1:10 | Gently ceded by Dr. Lucienne Pretto‐Giordano, UEL |
Abbreviations: BoHV‐1: bovine alphaherpesvirus type 1; BPIV‐3: bovine parainfluenza virus type 3; BRSV: bovine respiratory syncytial virus; BVDV: bovine viral diarrhea virus.