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. 2020 Jun;373(3):391–401. doi: 10.1124/jpet.119.264507

Fig. 8.

Fig. 8.

Effects of 4TFMPG on EA1-associated KCNA1 mutant channels All error bars indicate S.E.M. (A) Effects of 4TFMPG (100 µM) on KCNA1-E238K; n = 6. Left, mean traces; right, peak prepulse current vs. voltage. (B) Mean (left) and normalized (G/Gmax) (right) KCNA1-E283K tail current vs. prepulse voltage relationships calculated from traces as in (A); n = 6. Red dashed line, superimposed wild-type KCNA1 G/V curve (from Fig. 5D), baseline-adjusted to match that of the mutant channels. (C) Effects of 4TFMPG (100 µM) on resting membrane potential (EM) of unclamped X. laevis oocytes expressing KCNA1-E283K; n = 6. Red dashed line, baseline EM of wild-type KCNA1 for comparison (from Fig. 5F). (D) Mean traces showing effects of 4TFMPG (100 µM) on heteromeric KCNA1/KCNA1-E238K; n = 6. (E) Peak prepulse current vs. voltage for cells as in (D) (n = 6). (F) Effects of 4TFMPG (100 µM) on resting membrane potential (EM) of unclamped X. laevis oocytes expressing KCNA1/KCNA1-E283K; n = 6. Red dashed line, baseline EM of wild-type KCNA1 for comparison (from Fig. 5F). (G) Mean traces showing effects of 4TFMPG (100 µM) on KCNA1-V408A; n = 6. (H) Left, mean KCNA1-V408A tail currents vs. prepulse voltage relationships calculated from traces as in (G); n = 6. Red dashed line, superimposed normalized wild-type KCNA1 G/V curve (from Fig. 5D). Right, effects of 4TFMPG (100 µM) on EM of unclamped X. laevis oocytes expressing KCNA1-V408A; n = 6. Red dashed line, baseline EM of wild-type KCNA1 for comparison (from Fig. 5F). (I) Effects of 4TFMPG (100 µM) on homomeric KCNA1-G311D; left, mean traces; right, EM; n = 6. Red dashed line, baseline EM of wild-type KCNA1 for comparison (from Fig. 5F). (J) Effects of 4TFMPG (100 µM) on heteromeric KCNA1/KCNA1-G311D; left, mean traces; right, EM; n = 6. Red dashed line, baseline EM of wild-type KCNA1 for comparison (from Fig. 5F). (K) Effects of 4TFMPG (100 µM) on homomeric KCNA1/KCNA1-L328V; left, mean traces; right, EM; n = 5. (L) Effects of 4TFMPG (100 µM) on heteromeric KCNA1/KCNA1-L328V; left, mean traces; right, EM; n = 5.