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. 2020 Jun;373(3):337–346. doi: 10.1124/jpet.119.264374

Fig. 1.

Fig. 1.

Conditional knockdown of Nox4 expression in long bones. (A) Model for PrxCre-mediated excision of exons 1 and 2 of the Nox4 gene. Indicated are genotyping amplicons for distinguishing the various Nox4 alleles. (B–D) The concentrations of Nox4 mRNA from the knockout (K/O) region, total Nox4 mRNA, and Nox2 relative to β-actin mRNA were determined in the femoral shaft and femoral bone marrow from 13-week-old mice by qRT-PCR. There were 5–11 mice per group. (E and F) The concentration of Nox4 mRNA was determined in liver and kidney aliquots from 32-week-old female mice. There were four mice per group. *P < 0.05; ***P < 0.001 vs. the Nox4 fl/fl genotype. PCR, polymerase chain reaction.