TABLE 1.

Oligonucleotide primers used in site-directed mutagenesis

Amino Acid	Oligos for Mutagenesis to A		Oligos for Mutagenesis to D
T135	Left primer	5′ tga​acg​gac​agg​ggc​tca​gcc​act​ggg 3′	5′ aag​tga​acg​gac​agg​gga​tca​gcc​act​ggg​agt​g 3′
	Right primer	5′ ccc​agt​ggc​tga​gcc​cct​gtc​cgt​tca 3′	5′ cac​tcc​cag​tgg​ctg​atc​ccc​tgt​ccg​ttc​act​t 3′
S221	Left primer	5′ ttg​tag​ttc​cag​aca​gcg​cca​tcc​tcc​ccc​cg 3′	5′ ttt​gta​gtt​cca​gac​atc​gcc​atc​ctc​ccc​ccg​c 3′
	Right primer	5′ cgg​ggg​gag​gat​ggc​gct​gtc​tgg​aac​tac​aa 3′	5′ gcg​ggg​gga​gga​tgg​cga​tgt​ctg​gaa​cta​caa​a 3′