FIGURE 4.

Determining the frequency of highly allosensitized human sera containing antibodies Specific for class I SLA molecules. Human sera with panel reactive antibodies greater than 80 were absorbed with WT pig RBC and incubated with PBMC collected from GGTA1 knockout or GGTA1/SLA1 knockout swine. Flow cytometry determined MFIs of binding to these cells for IgG (A) and IgM (B). Thirteen of 22 samples contained class I SLA-specific IgG, and 4 of 17 samples, contained IgM that reacted with class I SLA proteins. Horizontal dotted line appears at MFI = 2000.